Anti-POU5F1/OCT3/OCT4 Polyclonal Antibody (HX129014)

Name: Anti-POU5F1/OCT3/OCT4 Polyclonal Antibody
Brand: abinScience
SKU: HX129014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HX129014

Description

Anti-POU5F1/OCT3/OCT4 Polyclonal Antibody (HX129014) is a rabbit polyclonal antibody detecting POU5F1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Macaca mulatta, and Pan troglodytes.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human POU5F1/OCT3/OCT4 (Ser136-Ser289).

Target

Octamer-binding protein 4, Oct-4, OCT3, OCT4, OTF3, OTF-3, Octamer-binding transcription factor 3, POU5F1, Oct-3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3

Purification

Purified by antigen affinity column.

Accession

Q01860

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

POU domain, class 5, transcription factor 1 (POU5F1/OCT3) is a ~38 kDa protein. Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-POU5F1/OCT3/OCT4 polyclonal antibody at 1ug/mL on recombinant POU5F1/OCT3/OCT4 (Catalog No. HX129012).

References

Datasheet

Anti-POU5F1/OCT3/OCT4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-POU5F1/OCT3/OCT4 Polyclonal Antibody (HX129014)

Overview

Images

References

Datasheet

SDS

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