Anti-PPARA Polyclonal Antibody (HC464014)

Name: Anti-PPARA Polyclonal Antibody
Brand: abinScience
SKU: HC464014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC464014

Description

Anti-PPARA Polyclonal Antibody (HC464014) is a rabbit polyclonal antibody detecting PPARA in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Cavia porcellus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPARA (Pro61-Ala170).

Target

Peroxisome proliferator-activated receptor alpha, PPAR, Nuclear receptor subfamily 1 group C member 1, PPAR-alpha, PPARA, NR1C1

Purification

Purified by antigen affinity column.

Accession

Q07869

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Peroxisome proliferator-activated receptor alpha (PPARA) is a ~52 kDa protein. Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PPARA polyclonal antibody at 1ug/mL on recombinant PPARA (Catalog No. HC464012).

References

Datasheet

Anti-PPARA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PPARA Polyclonal Antibody (HC464014)

Overview

Images

References

Datasheet

SDS

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