Anti-PPARD Polyclonal Antibody (HX185014)

Name: Anti-PPARD Polyclonal Antibody
Brand: abinScience
SKU: HX185014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HX185014

Description

Anti-PPARD Polyclonal Antibody (HX185014) is a rabbit polyclonal antibody detecting PPARD in ELISA, IHC, WB. Suitable for Human, Mouse, and Dog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPARD (Leu260-Glu426).

Target

PPARD, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, NR1C2, NUC1, Peroxisome proliferator-activated receptor delta, PPAR-beta, PPAR-delta, PPARB, NUCI, Peroxisome proliferator-activated receptor beta

Purification

Purified by antigen affinity column.

Accession

Q03181

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Peroxisome proliferator-activated receptor delta (PPARD) is a ~49 kDa protein. Ligand-activated transcription factor key mediator of energy metabolism in adipose tissues. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids.

1. Zhou, N. et al. (2022) Cell metabolism 34, 1023-1041.e8. PMID: 35675826

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PPARD polyclonal antibody at 1ug/mL on recombinant PPARD (Catalog No. HX185012).

References

Datasheet

Anti-PPARD Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PPARD Polyclonal Antibody (HX185014)

Overview

Images

References

Datasheet

SDS

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