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Anti-PPID Polyclonal Antibody (HC322014)

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Overview
Catalog No.HC322014
Description
Anti-PPID Polyclonal Antibody (HC322014) is a rabbit polyclonal antibody detecting PPID in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PPID (Gln7-Pro206).
Target Rotamase D, 40 kDa peptidyl-prolyl cis-trans isomerase, PPID, Cyclophilin-40, Peptidyl-prolyl cis-trans isomerase D, PPIase D, CYP40, CYP-40, Cyclophilin-related protein, CYPD
Purification Purified by antigen affinity column.
Accession Q08752
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Peptidyl-prolyl cis-trans isomerase D (PPID) is a ~40 kDa protein. PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus.

1. Pirkl, F. et al. (2001) Journal of molecular biology 308, 795-806. PMID: 11350175
2. Davis, TL. et al. (2010) PLoS biology 8, e1000439. PMID: 20676357
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-PPID Polyclonal Antibody

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