Anti-PPP3CA Polyclonal Antibody (HC519014)

Name: Anti-PPP3CA Polyclonal Antibody
Brand: abinScience
SKU: HC519014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HC519014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPP3CA (Ile7-Ser301).

Target

CAM-PRP catalytic subunit, CNA, Calmodulin-dependent calcineurin A subunit alpha isoform, CALNA, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, PPP3CA

Purification

Purified by antigen affinity column.

Accession

Q08209

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein phosphatase 3 catalytic subunit alpha (PPP3CA) is a ~58 kDa protein. Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Many of the substrates contain a PxIxIT motif and/or a LxVP motif. In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation. In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion. Positively regulates the CACNA1B/CAV2.2-mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals.

1. Wang, Y. et al. (2005) The Journal of biological chemistry 280, 12683-9. PMID: 15671020

2. Cereghetti, GM. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 15803-8. PMID: 18838687

3. Kilka, S. et al. (2009) Biochemistry 48, 1900-10. PMID: 19154138

4. Grigoriu, S. et al. (2013) PLoS biology 11, e1001492. PMID: 23468591

5. Rydzanicz, M. et al. (2019) European journal of human genetics : EJHG 27, 61-69. PMID: 30254215

6. Li, H. et al. (2007) Journal of molecular biology 369, 1296-306. PMID: 17498738

7. Takeuchi, K. et al. (2007) Structure (London, England : 1993) 15, 587-97. PMID: 17502104

8. Li, H. et al. (2012) Nature structural & molecular biology 19, 337-45. PMID: 22343722

9. Sheftic, SR. et al. (2016) Scientific reports 6, 38920. PMID: 27974827

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PPP3CA polyclonal antibody at 1ug/mL on recombinant PPP3CA (Catalog No. HC519012).

References

Datasheet

Anti-PPP3CA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-PPP3CA Polyclonal Antibody (HC519014)

Overview

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References

Datasheet

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