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Anti-PPRV Nucleoprotein Polyclonal Antibody (VK143014)

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Overview
Catalog No.VK143014
Description
Anti-PPRV Nucleoprotein Polyclonal Antibody (VK143014) is a rabbit polyclonal antibody detecting Nucleoprotein. Suitable for Peste-des-petits-ruminants virus.
Species reactivityPeste-des-petits-ruminants virus (PPRV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant PPRV Nucleoprotein (Val34-Ser395).
Target N, NP, Nucleocapsid protein, Nucleoprotein, Protein N
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q08823
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Nucleoprotein is a ~58 kDa protein. Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P. Participates, together with P, in the formation of viral factories (viroplasms), which are large inclusions in the host cytoplasm where replication takes place.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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