Anti-pptT Polyclonal Antibody (JN126014)

Name: Anti-pptT Polyclonal Antibody
Brand: abinScience
SKU: JN126014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

JN126014

Description

Anti-pptT Polyclonal Antibody (JN126014) is a rabbit polyclonal antibody detecting 4'-phosphopantetheinyl transferase PptT; PPTase; 2.7.8.7; pptT; Rv2794c in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mycobacterium tuberculosis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant pptT (Met1-Leu227).

Target

4'-phosphopantetheinyl transferase PptT; PPTase; 2.7.8.7; pptT; Rv2794c

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O33336

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

4'-phosphopantetheinyl transferase PptT is a ~24 kDa protein. Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein. Involved in post-translational modification of various type-I polyketide synthases required for the formation of both mycolic acids and lipid virulence factors. Acts on Pks13, Mas, PpsA, PpsB, PpsC and PpsD. Also acts on AcpM, the meromycolate extension acyl carrier protein. In addition, is involved in the activation of the acyl carrier protein MbtL and the nonribosomal peptides synthases MbtB and MbtE, which are involved in the biosynthesis of the siderophore mycobactin.

1. Chalut, C. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 8511-6. PMID: 16709676

2. Zimhony, O. et al. (2015) Biochemistry 54, 2360-71. PMID: 25785780

3. Jung, J. et al. (2016) FEBS open bio 6, 1220-1226. PMID: 28203522

4. Quadri, LE. et al. (1998) Chemistry & biology 5, 631-45. PMID: 9831524

Note

For research use only

Images

References

Datasheet

Anti-pptT Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-pptT Polyclonal Antibody (JN126014)

Overview

Images

References

Datasheet

SDS

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