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Anti-PREP Polyclonal Antibody (HD430014)

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Anti-PREP Polyclonal Antibody
Anti-PREP Polyclonal Antibody
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Overview
Catalog No.HD430014
Description
Anti-PREP Polyclonal Antibody (HD430014) is a rabbit polyclonal antibody detecting PREP in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PREP (Ser77-Glu424).
Target EC:3.4.21.26, PE, PEP, PREP, Post-proline cleaving enzyme, Prolyl endopeptidase
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P48147
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesP48147
Background

Prolyl endopeptidase (PREP) is a ~80 kDa protein. Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.

Note For research use only
Images
  • Anti-PREP Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PREP polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Jurkat cell lysate
    Lane 2: HL-60 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-PREP Polyclonal Antibody

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