Anti-PRKAG1 Polyclonal Antibody (HX903014)

Name: Anti-PRKAG1 Polyclonal Antibody
Brand: abinScience
SKU: HX903014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HX903014

Description

Anti-PRKAG1 Polyclonal Antibody (HX903014) is a rabbit polyclonal antibody detecting PRKAG1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PRKAG1 (Arg70-Leu300).

Target

AMPK gamma1, AMPKg, AMPK subunit gamma-1, PRKAG1, 5'-AMP-activated protein kinase subunit gamma-1

Purification

Purified by antigen affinity column.

Accession

P54619

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

5'-AMP-activated protein kinase subunit gamma-1 (PRKAG1) is a ~37 kDa protein. AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits.

1. Oakhill, JS. et al. (2011) Science (New York, N.Y.) 332, 1433-5. PMID: 21680840

2. Bullen, JW. et al. (2014) The Journal of biological chemistry 289, 10592-10606. PMID: 24563466

Note

For research use only.

Images

References

Datasheet

Anti-PRKAG1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PRKAG1 Polyclonal Antibody (HX903014)

Overview

Images

References

Datasheet

SDS

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