Anti-PRKN Polyclonal Antibody (HT315014)

Name: Anti-PRKN Polyclonal Antibody
Brand: abinScience
SKU: HT315014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HT315014

Description

Anti-PRKN Polyclonal Antibody (HT315014) is a rabbit polyclonal antibody detecting PRKN in WB, IHC, ELISA. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

WB,IHC,ELISA

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human PRKN (Ile229-Val465).

Target

PRKN

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

O60260, Q9JK66, Q9WVS6

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

E3 ubiquitin-protein ligase parkin, Parkin, EC:2.3.2.31, Parkin RBR E3 ubiquitin-protein ligase, Parkinson juvenile disease protein 2, Parkinson disease protein 2, PRKN

Background

E3 ubiquitin-protein ligase parkin (PRKN) is a ~51 kDa protein. Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Substrates include SYT11 and VDAC1. Other substrates are BCL2, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPTIN5, TOMM20, USP30, ZNF746, MIRO1 and AIMP2. Mediates monoubiquitination as well as 'Lys-6', 'Lys-11', 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation.

1. Shimura, H. et al. (2000) Nature genetics 25, 302-5. PMID: 10888878

2. Imai, Y. et al. (2000) The Journal of biological chemistry 275, 35661-4. PMID: 10973942

3. Shimura, H. et al. (2001) Science (New York, N.Y.) 293, 263-9. PMID: 11431533

4. Imai, Y. et al. (2002) Molecular cell 10, 55-67. PMID: 12150907

5. Staropoli, JF. et al. (2003) Neuron 37, 735-49. PMID: 12628165

6. Chung, KK. et al. (2004) Science (New York, N.Y.) 304, 1328-31. PMID: 15105460

7. Ko, HS. et al. (2005) The Journal of neuroscience : the official journal of the Society for Neuroscience 25, 7968-78. PMID: 16135753

8. Shin, JH. et al. (2011) Cell 144, 689-702. PMID: 21376232

9. Wenzel, DM. et al. (2011) Nature 474, 105-8. PMID: 21532592

10. Liu, S. et al. (2012) PLoS genetics 8, e1002537. PMID: 22396657

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PRKN polyclonal antibody at 1ug/mL on recombinant PRKN (Catalog No. HT315012).

References

Datasheet

Anti-PRKN Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-PRKN Polyclonal Antibody (HT315014)

Overview

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References

Datasheet

SDS

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