Anti-PSMB3 Polyclonal Antibody (HA658014)

Name: Anti-PSMB3 Polyclonal Antibody
Brand: abinScience
SKU: HA658014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA658014

Description

Anti-PSMB3 Polyclonal Antibody (HA658014) is a rabbit polyclonal antibody detecting Proteasome subunit beta type-3; Proteasome chain 13; Proteasome component C10-II; Proteasome theta chain; PSMB3 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human PSMB3 (Met1-Asp205).

Target

Proteasome subunit beta type-3; Proteasome chain 13; Proteasome component C10-II; Proteasome theta chain; PSMB3

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P49720, Q9R1P1, P33672, P40112

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Proteasome subunit beta type-3 (PSMB3) is a ~22 kDa protein. Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-PSMB3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: NIH3T3 cell lysate

References

Datasheet

Anti-PSMB3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PSMB3 Polyclonal Antibody (HA658014)

Overview

Images

References

Datasheet

SDS