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Anti-PSMC6 Polyclonal Antibody (HX951014)

Anti-PSMC6 Polyclonal Antibody
Anti-PSMC6 Polyclonal Antibody
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Overview
Catalog No.HX951014
Description
Anti-PSMC6 Polyclonal Antibody (HX951014) is a rabbit polyclonal antibody detecting PSMC6 in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PSMC6 (Met1-Val389).
Target SUG2, 26S proteasome AAA-ATPase subunit RPT4, 26S proteasome regulatory subunit 10B, Proteasome subunit p42, Proteasome 26S subunit ATPase 6, PSMC6
Purification Purified by antigen affinity column.
Accession P62333
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

26S proteasome regulatory subunit 10B (PSMC6) is a ~44 kDa protein. Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC6 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

Note For research use only.
Images
  • Anti-PSMC6 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PSMC6 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: 293T cell lysate
    Lane 2: SH-SY5Y cell lysate
    Lane 3: NCI-H460 cell lysate
    Lane 4: Hela cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: Mouse brain lysate
    Lane 7: Mouse liver lysate
    Lane 8: Mouse spleen lysate
    Lane 9: Mouse kidney lysate
    Lane 10: Rat brain lysate
    Lane11: Rat liver lysate
    Lane12: Rat spleen lysate
    Lane13: Rat kidney lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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