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Anti-PTGIS Polyclonal Antibody (MS772014)

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Overview
Catalog No.MS772014
Description
Anti-PTGIS Polyclonal Antibody (MS772014) is a rabbit polyclonal antibody detecting Prostacyclin synthase; 5.3.99.4; Hydroperoxy icosatetraenoate dehydratase; 4.2.1.152; Prostaglandin I2 synthase; Ptgis; Cyp8 in ELISA, IHC, WB. Suitable for Mouse, Human, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant mouse PTGIS (Gly42-Leu501).
Target Prostacyclin synthase; 5.3.99.4; Hydroperoxy icosatetraenoate dehydratase; 4.2.1.152; Prostaglandin I2 synthase; Ptgis; Cyp8
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q16647, O35074, Q62969
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Prostacyclin synthase (PTGIS) is a ~57 kDa protein. Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE).

1. Nakayama, T. et al. (2002) Biochemical and biophysical research communications 297, 1135-9. PMID: 12372404
2. Wada, M. et al. (2004) Journal of biochemistry 135, 455-63. PMID: 15115769
3. Li, YC. et al. (2008) The Journal of biological chemistry 283, 2917-26. PMID: 18032380
4. Cho, SA. et al. (2015) Archives of biochemistry and biophysics 569, 10-8. PMID: 25623425
5. Yeh, HC. et al. (2007) Archives of biochemistry and biophysics 461, 159-68. PMID: 17459323
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-PTGIS Polyclonal Antibody

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