Anti-PXDN Polyclonal Antibody (HV750014)

Name: Anti-PXDN Polyclonal Antibody
Brand: abinScience
SKU: HV750014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HV750014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PXDN (Gln1285-Ala1475).

Target

Peroxidasin homolog, VPO1, MG50, p53-responsive gene 2 protein, Vascular peroxidase 1, KIAA0230, Melanoma-associated antigen MG50, VPO, PXDN, PRG2

Purification

Purified by antigen affinity column.

Accession

Q92626

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Peroxidasin homolog (PXDN) is a ~165 kDa protein. Catalyzes the two-electron oxidation of bromide by hydrogen peroxide and generates hypobromite as a reactive intermediate which mediates the formation of sulfilimine cross-links between methionine and hydroxylysine residues within an uncross-linked collagen IV/COL4A1 NC1 hexamer. In turns, directly contributes to the collagen IV network-dependent fibronectin/FN and laminin assembly, which is required for full extracellular matrix (ECM)-mediated signaling. Thus, sulfilimine cross-links are essential for growth factor-induced cell proliferation and survival in endothelial cells, an event essential to basement membrane integrity. In addition, through the bromide oxidation, may promote tubulogenesis and induce angiogenesis through ERK1/2, Akt, and FAK pathways. Moreover brominates alpha2 collagen IV chain/COL4A2 at 'Tyr-1485' and leads to bromine enrichment of the basement membranes.

1. Cheng, G. et al. (2008) Free radical biology & medicine 45, 1682-94. PMID: 18929642

2. Péterfi, Z. et al. (2009) The American journal of pathology 175, 725-35. PMID: 19590037

3. Bhave, G. et al. (2012) Nature chemical biology 8, 784-90. PMID: 22842973

4. Lázár, E. et al. (2015) Free radical biology & medicine 83, 273-82. PMID: 25708780

5. Soudi, M. et al. (2015) The Journal of biological chemistry 290, 10876-90. PMID: 25713063

6. Colon, S. et al. (2016) The Journal of biological chemistry 291, 24009-24016. PMID: 27697841

7. Paumann-Page, M. et al. (2017) The Journal of biological chemistry 292, 4583-4592. PMID: 28154175

8. Kovács, HA. et al. (2021) Antioxidants (Basel, Switzerland) 10. PMID: 34679700

9. Lee, SW. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 10228-10241. PMID: 32543734

10. He, C. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 15827-15836. PMID: 32571911

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PXDN polyclonal antibody at 1ug/mL on recombinant PXDN (Catalog No. HV750012).

References

Datasheet

Anti-PXDN Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PXDN Polyclonal Antibody (HV750014)

Overview

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References

Datasheet

SDS

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