ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
23.75 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Peroxidasin homolog (PXDN) is a ~165 kDa protein. Catalyzes the two-electron oxidation of bromide by hydrogen peroxide and generates hypobromite as a reactive intermediate which mediates the formation of sulfilimine cross-links between methionine and hydroxylysine residues within an uncross-linked collagen IV/COL4A1 NC1 hexamer. In turns, directly contributes to the collagen IV network-dependent fibronectin/FN and laminin assembly, which is required for full extracellular matrix (ECM)-mediated signaling. Thus, sulfilimine cross-links are essential for growth factor-induced cell proliferation and survival in endothelial cells, an event essential to basement membrane integrity. In addition, through the bromide oxidation, may promote tubulogenesis and induce angiogenesis through ERK1/2, Akt, and FAK pathways. Moreover brominates alpha2 collagen IV chain/COL4A2 at 'Tyr-1485' and leads to bromine enrichment of the basement membranes.
1. Cheng, G. et al. (2008) Free radical biology & medicine 45, 1682-94. PMID: 18929642
2. Péterfi, Z. et al. (2009) The American journal of pathology 175, 725-35. PMID: 19590037
3. Bhave, G. et al. (2012) Nature chemical biology 8, 784-90. PMID: 22842973
4. Lázár, E. et al. (2015) Free radical biology & medicine 83, 273-82. PMID: 25708780
5. Soudi, M. et al. (2015) The Journal of biological chemistry 290, 10876-90. PMID: 25713063
6. Colon, S. et al. (2016) The Journal of biological chemistry 291, 24009-24016. PMID: 27697841
7. Paumann-Page, M. et al. (2017) The Journal of biological chemistry 292, 4583-4592. PMID: 28154175
8. Kovács, HA. et al. (2021) Antioxidants (Basel, Switzerland) 10. PMID: 34679700
9. Lee, SW. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 10228-10241. PMID: 32543734
10. He, C. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 15827-15836. PMID: 32571911
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human PXDN Protein, N-His
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