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Anti-PYGB Polyclonal Antibody (HY073014)

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Overview
Catalog No.HY073014
Description
Anti-PYGB Polyclonal Antibody (HY073014) is a rabbit polyclonal antibody detecting PYGB in ELISA, IHC, WB. Suitable for Rat, Bovine, Human, Mouse, and Ovis aries.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PYGB (Pro477-Glu731).
Target Glycogen phosphorylase, brain form, PYGB
Purification Purified by antigen affinity column.
Accession P11216
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Glycogen phosphorylase, brain form (PYGB) is a ~96 kDa protein. Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

1. Mathieu, C. et al. (2016) The Journal of biological chemistry 291, 18072-83. PMID: 27402852
2. Newgard, CB. et al. (1988) The Journal of biological chemistry 263, 3850-7. PMID: 3346228
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-PYGB Polyclonal Antibody

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