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Anti-RAB5C Polyclonal Antibody (HB399014)

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Overview
Catalog No.HB399014
Description
Anti-RAB5C Polyclonal Antibody (HB399014) is a rabbit polyclonal antibody detecting Ras-related protein Rab-5C; 3.6.5.2; L1880; RAB5L; RAB5C; RABL in ELISA, IHC, WB. Suitable for Mouse, Dog, Human, Bovine, and Chicken.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human RAB5C (Asn17-Leu182).
Target Ras-related protein Rab-5C; 3.6.5.2; L1880; RAB5L; RAB5C; RABL
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P35278, P51147, P51148, Q58DS9, Q98932, Q9CQD1, Q06AU6, Q0IIG7, P20339, P18066, M0RC99, Q5ZHW4, P61020, P61021
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ras-related protein Rab-5C (RAB5C) is a ~23 kDa protein. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Involved in early endocytic trafficking. Required for EEA1 recruitment to early endosomes. Required for EGF and transferrin endocytosis and trafficking through early endosomes.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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