Anti-RAB7A Polyclonal Antibody (HW603014)

Name: Anti-RAB7A Polyclonal Antibody
Brand: abinScience
SKU: HW603014
Price: 96 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HW603014

Description

Anti-RAB7A Polyclonal Antibody (HW603014) is a rabbit polyclonal antibody detecting RAB7A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Dog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Bos taurus, Dog, Epinephelus coioides, Oryctolagus cuniculus, Pongo abelii

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RAB7A (Met1-Cys207).

Target

RAB7A, RAB7, Ras-related protein Rab-7a

Purification

Purified by antigen affinity column.

Accession

P51149

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ras-related protein Rab-7a (RAB7A) is a ~23 kDa protein. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. In its active state, RAB7A binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Also plays a central role in growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism.

1. Li, L. et al. (2024) Nature 628, 630-638. PMID: 38538795

2. Cantalupo, G. et al. (2001) The EMBO journal 20, 683-93. PMID: 11179213

3. Harrison, RE. et al. (2003) Molecular and cellular biology 23, 6494-506. PMID: 12944476

4. Stein, MP. et al. (2003) Traffic (Copenhagen, Denmark) 4, 754-71. PMID: 14617358

5. McCray, BA. et al. (2010) Human molecular genetics 19, 1033-47. PMID: 20028791

6. Seto, S. et al. (2011) Traffic (Copenhagen, Denmark) 12, 407-20. PMID: 21255211

7. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413

8. Daniloski, Z. et al. (2021) Cell 184, 92-105.e16. PMID: 33147445

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RAB7A polyclonal antibody at 1ug/mL on recombinant RAB7A (Catalog No. HW603012).

References

Datasheet

Anti-RAB7A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-RAB7A Polyclonal Antibody (HW603014)

Overview

Images

References

Datasheet

SDS

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