Anti-RAC1 Polyclonal Antibody (HX934014)

Name: Anti-RAC1 Polyclonal Antibody
Brand: abinScience
SKU: HX934014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HX934014

Description

Anti-RAC1 Polyclonal Antibody (HX934014) is a rabbit polyclonal antibody detecting RAC1 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RAC1 (Met1-Leu192).

Target

Ras-related C3 botulinum toxin substrate 1, Ras-like protein TC25, TC25, p21-Rac1, Cell migration-inducing gene 5 protein, RAC1

Purification

Purified by antigen affinity column.

Accession

P63000

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ras-related C3 botulinum toxin substrate 1 (RAC1) is a ~21 kDa protein. Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity.

1. Ridley, AJ. et al. (1992) Cell 70, 401-10. PMID: 1643658

2. Hong, IK. et al. (2012) The Journal of biological chemistry 287, 32027-39. PMID: 22843693

3. Zhao, J. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 2611-9. PMID: 23512198

4. Reijnders, MRF. et al. (2017) American journal of human genetics 101, 466-477. PMID: 28886345

5. Vincent, S. et al. (1997) Molecular and cellular biology 17, 2247-56. PMID: 9121475

6. Zhang, H. et al. (2003) The Journal of cell biology 161, 131-42. PMID: 12695502

7. Tsang, SM. et al. (2012) Experimental cell research 318, 2269-83. PMID: 22796473

8. Liu, X. et al. (2024) Nature 627, 189-195. PMID: 38355798

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RAC1 polyclonal antibody at 1ug/mL on recombinant RAC1 (Catalog No. HX934012).

References

Datasheet

Anti-RAC1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-RAC1 Polyclonal Antibody (HX934014)

Overview

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References

Datasheet

SDS

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