Anti-RAD21 Polyclonal Antibody (HA549014)

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Overview

Catalog No.

HA549014

Description

Anti-RAD21 Polyclonal Antibody (HA549014) is a rabbit polyclonal antibody detecting RAD21 in WB, IHC, ELISA. Suitable for Human, Mus musculus, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Homo sapiens, Mus musculus, Bos taurus

Applications

WB, IHC, ELISA

Host species

Rabbit

Isotype

IgG

Species

Homo sapiens (Human)

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant RAD21 (Glu147-Thr349).

Target

RAD21

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

O60216

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, NXP-1, SCC1 homolog, 64-kDa C-terminal product, 64-kDa carboxy-terminal product, 65-kDa carboxy-terminal product, RAD21

Background

Double-strand-break repair protein rad21 homolog is a ~71 kDa protein. As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions. The cohesin complex may also play a role in spindle pole assembly during mitosis. In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome. May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter.

1. Hauf, S. et al. (2001) Science (New York, N.Y.) 293, 1320-3. PMID: 11509732

2. Gregson, HC. et al. (2001) The Journal of biological chemistry 276, 47575-82. PMID: 11590136

3. Bonora, E. et al. (2015) Gastroenterology 148, 771-782.e11. PMID: 25575569

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RAD21 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: K562 cell lysate

References

Datasheet

Anti-RAD21 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-RAD21 Polyclonal Antibody (HA549014)

Overview

Images

References

Datasheet

SDS

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