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Anti-RALA Polyclonal Antibody (HY266014)

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Overview
Catalog No.HY266014
Description
Anti-RALA Polyclonal Antibody (HY266014) is a rabbit polyclonal antibody detecting RALA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Saguinus oedipus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human RALA (Met1-Leu206).
Target Ras-related protein Ral-A, RAL, RALA
Purification Purified by antigen affinity column.
Accession P11233
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ras-related protein Ral-A (RALA) is a ~23 kDa protein. Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor.

1. Cascone, I. et al. (2008) The EMBO journal 27, 2375-87. PMID: 18756269
2. Aziziyeh, AI. et al. (2009) Cellular signalling 21, 1207-17. PMID: 19306925
3. Balasubramanian, N. et al. (2010) Current biology : CB 20, 75-9. PMID: 20005108
4. Kashatus, DF. et al. (2011) Nature cell biology 13, 1108-15. PMID: 21822277
5. Hiatt, SM. et al. (2018) PLoS genetics 14, e1007671. PMID: 30500825
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-RALA Polyclonal Antibody

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