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Anti-Rat AADAT Polyclonal Antibody (RA570014)

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Overview
Catalog No.RA570014
Description
Anti-Rat AADAT Polyclonal Antibody (RA570014) is a rabbit polyclonal antibody detecting AadAT in ELISA, IHC, WB. Suitable for Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityRat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Rat AADAT (Pro30-Ser425).
Target Kynurenine/alpha-aminoadipate aminotransferase mitochondrial, KAT/AadAT, 2-aminoadipate aminotransferase, 2-aminoadipate transaminase, EC:2.6.1.39, Alpha-aminoadipate aminotransferase, AadAT, Glycine transaminase AADAT, EC:2.6.1.4, Kynurenine aminotransferase II, Kynurenine--glyoxylate transaminase AADAT, EC:2.6.1.63, Kynurenine--oxoglutarate aminotransferase II, Kynurenine--oxoglutarate transaminase 2, EC:2.6.1.7, Kynurenine--oxoglutarate transaminase II, Methionine--glyoxylate transaminase AADAT, EC:2.6.1.73, AADAT
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession Q64602
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (AADAT) is a ~47 kDa protein. Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).

Note For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Rat AADAT Polyclonal Antibody

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