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Anti-Rat C1QBP Polyclonal Antibody (RC372014)

Anti-Rat C1QBP Polyclonal Antibody
Anti-Rat C1QBP Polyclonal Antibody
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Overview
Catalog No.RC372014
Description
Anti-Rat C1QBP Polyclonal Antibody (RC372014) is a rabbit polyclonal antibody detecting C1QBP in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, and Bovine.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Rat C1QBP (Leu72-Gln279(Thr210Ala)).
Target C1QBP
Purification Purified by antigen affinity column.
Accession O35796
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesComplement component 1 Q subcomponent-binding protein, mitochondrial, GC1q-R protein, Glycoprotein gC1qBP, C1qBP, C1qbp, Gc1qbp
Background

Complement component 1 Q subcomponent-binding protein, mitochondrial (C1QBP) is a ~30 kDa protein. Multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, protein synthesis in mitochondria, regulation of apoptosis, transcriptional regulation and pre-mRNA splicing. At the cell surface is thought to act as an endothelial receptor for plasma proteins of the complement and kallikrein-kinin cascades. Putative receptor for C1q; specifically binds to the globular 'heads' of C1q thus inhibiting C1; may perform the receptor function through a complex with C1qR/CD93. In complex with cytokeratin-1/KRT1 is a high affinity receptor for kininogen-1/HMWK. Can also bind other plasma proteins, such as coagulation factor XII leading to its autoactivation.

Note For research use only.
Images
  • Anti-Rat C1QBP Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-C1QBP polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: K562 cell lysate
    Lane 4: PC-12 cell lysate
    Lane 5: C2C12 cell lysate
    Lane 6: A549 cell lysate
    Lane 7: Mouse spleen lysate
    Lane 8: Mouse lung lysate
    Lane 9: Rat spleen lysate
    Lane 10: Rat lung lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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