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Anti-Rat Aminopeptidase N/CD13 Polyclonal Antibody (RB650014)

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Anti-Rat Aminopeptidase N/CD13 Polyclonal Antibody
Anti-Rat Aminopeptidase N/CD13 Polyclonal Antibody
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Overview
Catalog No.RB650014
Description
Anti-Rat CD13/ANPEP/APN Polyclonal Antibody (RB650014) is a rabbit polyclonal antibody detecting ANPEP in ELISA, IHC, WB. Suitable for Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityRat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Rat CD13/ANPEP/APN (Thr75-Ser280).
Tested applications WB
Target ANPEP, AP-M, AP-N, APN, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, CD13, EC:3.4.11.2, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, PEPN, gp150, hAPN
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P15684
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Aminopeptidase N (CD13/ANPEP) is a ~108 kDa protein. Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. It is able to degrade Leu-enkephalin and Met-enkephalin but not cholecystokinin CCK8, neuromedin C (GRP-10), somatostatin-14, substance P and vasoactive intestinal peptide.

1. Terashima, H. et al. (1995) Journal of gastroenterology 30, 696-704. PMID: 8963385
Note For research use only.
Images
  • Anti-Rat Aminopeptidase N/CD13 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ANPEP polyclonal antibody at 1ug/mL on various samples.
    Lane 1: PC-3 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Rat Aminopeptidase N/CD13 Polyclonal Antibody

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