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Anti-Rat Mbl1 Polyclonal Antibody (RP768014)

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Overview
Catalog No.RP768014
Description
Anti-Rat Mbl1 Polyclonal Antibody (RP768014) is a rabbit polyclonal antibody detecting Mbl1 in ELISA, IHC, WB. Suitable for Rat.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityRat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Rat Mbl1 (Gly86-Ala238).
Target Mannose-binding protein A, MBP-A, Mannan-binding protein, Mbl1
Purification Purified by antigen affinity column.
Accession P19999
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Mannose-binding protein A (Mbl1) is a ~25 kDa protein. Calcium-dependent lectin. Plays a role in the innate immune response by binding mannose, fucose and N-acetylglucosamine moieties on different microorganisms and mediating activation of the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages.

1. Ng, KK. et al. (2002) The Journal of biological chemistry 277, 16088-95. PMID: 11850428
2. Weis, WI. et al. (1992) Nature 360, 127-34. PMID: 1436090
3. Ng, KK. et al. (1997) Biochemistry 36, 979-88. PMID: 9033386
4. Ikeda, K. et al. (1987) The Journal of biological chemistry 262, 7451-4. PMID: 3584121
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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