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Anti-RBBP5 Polyclonal Antibody (HS761014)

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Overview
Catalog No.HS761014
Description
Anti-RBBP5 Polyclonal Antibody (HS761014) is a rabbit polyclonal antibody detecting Retinoblastoma-binding protein 5; RBBP-5; Retinoblastoma-binding protein RBQ-3; RBBP5; RBQ3 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human RBBP5 (Glu15-Asn324).
Target Retinoblastoma-binding protein 5; RBBP-5; Retinoblastoma-binding protein RBQ-3; RBBP5; RBQ3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q15291, Q8BX09
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Retinoblastoma-binding protein 5 (RBBP5) is a ~59 kDa protein. In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal. Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters. As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.

1. Garapaty, S. et al. (2009) The Journal of biological chemistry 284, 7542-52. PMID: 19131338
2. Patel, A. et al. (2009) The Journal of biological chemistry 284, 24242-56. PMID: 19556245
3. Avdic, V. et al. (2011) Structure (London, England : 1993) 19, 101-8. PMID: 21220120
4. Zhang, P. et al. (2012) Nucleic acids research 40, 4237-46. PMID: 22266653
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-RBBP5 Polyclonal Antibody

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