Anti-RBD (Omicron/B.1.1.529) Polyclonal Antibody (VK565024) is a rabbit polyclonal antibody detecting RBD (Omicron/B.1.1.529) in ELISA, IHC, WB.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
Species reactivity
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Applications
ELISA, IHC, WB
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant RBD (Omicron/B.1.1.529) (Arg316-Phe538).
Tested applications
WB
Target
surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], S, N-terminal domain of the S1 subunit of the Spike (S) protein from Severe acute respiratory syndrome coronavirus and related betacoronaviruses in the B lineage; cd21624, trimer interface [polypeptide binding], receptor-binding domain of the S1 subunit of coronavirus spike (S) proteins; cl40478, trimer interface [polypeptide binding], putative receptor binding site [polypeptide binding], receptor binding motif, SD-1 and SD-2 subdomains, the S1/S2 cleavage region, and the S2 fusion subunit of the spike (S) glycoprotein from SARS-CoV-2 (COVID-19) and related betacoronaviruses in the B lineage; cd22378, N-linked glycosylation sites [posttranslational modification], S1/S2 cleavage region, S1/S2 furin cleavage site, fusion peptide, internal fusion peptide, heptad repeat 1 [structural motif], heptad repeat 2 [structural motif]
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application
Dilution
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Spike glycoprotein (RBD) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors.
1. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8. PMID: 32142651
7. Wang, J. et al. (2021) Cell discovery 7, 119. PMID: 34903715
8. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 36779763
9. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 33082294
10. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293
Note
For research use only
Images
Western blot
Western blot analysis was performed using anti-RBD polyclonal antibody at 1ug/mL on various samples. Lane 1: recombinant RBD (Omicron/B.1.1.529) (Catalog No: VK565022)
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