Anti-RBM25 Polyclonal Antibody (HB412014)

Name: Anti-RBM25 Polyclonal Antibody
Brand: abinScience
SKU: HB412014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HB412014

Description

Anti-RBM25 Polyclonal Antibody (HB412014) is a rabbit polyclonal antibody detecting RNA-binding protein 25; Arg/Glu/Asp-rich protein of 120 kDa; RED120; Protein S164; RNA-binding motif protein 25; RNA-binding region-containing protein 7; RBM25; RNPC7 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human RBM25 (Thr86-Ala224).

Target

RNA-binding protein 25; Arg/Glu/Asp-rich protein of 120 kDa; RED120; Protein S164; RNA-binding motif protein 25; RNA-binding region-containing protein 7; RBM25; RNPC7

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P49756, B2RY56

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

RNA-binding protein 25 (RBM25) is a ~100 kDa protein. RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-RBM25 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: NIH3T3 cell lysate

References

Datasheet

Anti-RBM25 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-RBM25 Polyclonal Antibody (HB412014)

Overview

Images

References

Datasheet

SDS

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