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Anti-RBM33 Polyclonal Antibody (HB545014)

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Overview
Catalog No.HB545014
Description
Anti-RBM33 Polyclonal Antibody (HB545014) is a rabbit polyclonal antibody detecting RNA-binding protein 33; Proline-rich protein 8; RNA-binding motif protein 33; RBM33; PRR8 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human RBM33 (Gln1096-Glu1170).
Target RNA-binding protein 33; Proline-rich protein 8; RNA-binding motif protein 33; RBM33; PRR8
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q96EV2, Q9CXK9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

RNA-binding protein 33 (RBM33) is a ~129 kDa protein. RNA reader protein, which recognizes and binds specific RNAs, thereby regulating RNA metabolic processes, such as mRNA export, mRNA stability and/or translation. Binds a subset of intronless RNAs containing GC-rich elements, such as NORAD, and promotes their nuclear export by recruiting target RNAs to components of the NXF1-NXT1 RNA export machinery. Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, promoting their demethylation by ALKBH5. Acts as an molecular adapter, which (1) promotes ALKBH5 recruitment to m6A-containing transcripts and (2) activates ALKBH5 demethylase activity by recruiting SENP1, leading to ALKBH5 deSUMOylation and subsequent activation.

1. Thomas, A. et al. (2022) Genes & development 36, 550-565. PMID: 35589130
2. Yu, F. et al. (2023) Molecular cell 83, 2003-2019.e6. PMID: 37257451
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-RBM33 Polyclonal Antibody

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