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Anti-REBOV Nucleoprotein Polyclonal Antibody (VK518014)

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Overview
Catalog No.VK518014
Description
Anti-REBOV Nucleoprotein Polyclonal Antibody (VK518014) is a rabbit polyclonal antibody detecting nucleoprotein in ELISA, IHC, WB. Suitable for Sudan ebolavirus, Reston ebolavirus, and Zaire ebolavirus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivitySudan ebolavirus (SEBOV) (Sudan Ebola virus), Reston ebolavirus (REBOV) (Reston Ebola virus), Zaire ebolavirus (ZEBOV) (Zaire Ebola virus)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant REBOV Nucleoprotein (Val36-Asn416).
Target nucleoprotein, NP
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q5XX08, Q9QP77, Q8JPY1, Q91DE1, O72142, P18272, Q9QCE9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nucleoprotein is a ~81 kDa protein. Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm.

Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-REBOV Nucleoprotein Polyclonal Antibody

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