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Anti-RENBP Polyclonal Antibody (HW295014)

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Overview
Catalog No.HW295014
Description
Anti-RENBP Polyclonal Antibody (HW295014) is a rabbit polyclonal antibody detecting RENBP in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Pig.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human RENBP (Met11-Gln254).
Target Renin-binding protein, RnBP, RENBP, N-acetyl-D-glucosamine 2-epimerase, GlcNAc 2-epimerase, N-acylglucosamine 2-epimerase, AGE
Purification Purified by antigen affinity column.
Accession P51606
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

N-acylglucosamine 2-epimerase (RENBP) is a ~48 kDa protein. Catalyzes the interconversion of N-acetylglucosamine to N-acetylmannosamine. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway: although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded.

1. Takahashi, S. et al. (1999) Journal of biochemistry 126, 639-42. PMID: 10502668
2. Luchansky, SJ. et al. (2003) The Journal of biological chemistry 278, 8035-42. PMID: 12499362
3. Takahashi, S. et al. (1999) Journal of biochemistry 125, 348-53. PMID: 9990133
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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