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Anti-RFC3 Polyclonal Antibody (HD025014)

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Anti-RFC3 Polyclonal Antibody
Anti-RFC3 Polyclonal Antibody
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Overview
Catalog No.HD025014
Description
Anti-RFC3 Polyclonal Antibody (HD025014) is a rabbit polyclonal antibody detecting RFC3 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human RFC3 (Met1-Phe356).
Target A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, RF-C 38 kDa subunit, RFC3, RFC38, Replication factor C 38 kDa subunit, Replication factor C subunit 3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P40938
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesP40938
Background

Replication factor C subunit 3 (RFC3) is a ~40 kDa protein. Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA.

Note For research use only
Images
  • Anti-RFC3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RFC3 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: K562 cell lysate
    Lane 5: Lncap cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-RFC3 Polyclonal Antibody

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