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Anti-RHO Polyclonal Antibody (HY021014)

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Overview
Catalog No.HY021014
Description
Anti-RHO Polyclonal Antibody (HY021014) is a rabbit polyclonal antibody detecting Rhodopsin; Opsin-2; RHO; OPN2 in ELISA, IHC, WB. Suitable for Human, Dog, Pig, Cat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human RHO (Asn2-Gln36&Thr97-Cys110&Gly174-Ser202).
Target Rhodopsin; Opsin-2; RHO; OPN2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P08100, P32308, O18766, Q95KU1, P15409, P02700, P51489, P02699, P29403, P28683, P49912
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Rhodopsin is a ~38 kDa protein. Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.

1. Sieving, PA. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 880-4. PMID: 7846071
2. Rao, VR. et al. (1994) Nature 367, 639-42. PMID: 8107847
3. Noorwez, SM. et al. (2003) The Journal of biological chemistry 278, 14442-14450. PMID: 12566452
4. Dryja, TP. et al. (1990) The New England journal of medicine 323, 1302-7. PMID: 2215617
5. Kang, Y. et al. (2015) Nature 523, 561-7. PMID: 26200343
6. He, Y. et al. (2017) Cell research 27, 728-747. PMID: 28524165
7. Zhou, XE. et al. (2017) Cell 170, 457-469.e13. PMID: 28753425
Note For research use only
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Datasheet
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Formula
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Anti-RHO Polyclonal Antibody

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