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Anti-Rice ABI5 Polyclonal Antibody (PR141014)

Anti-Rice ABI5 Polyclonal Antibody
Anti-Rice ABI5 Polyclonal Antibody
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Overview
Catalog No.PR141014
Description
Anti-Rice ABI5 Polyclonal Antibody (PR141014) is a rabbit polyclonal antibody detecting ABI5 in ELISA, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
Species reactivityOryza sativa (Rice)
ApplicationsELISA, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Rice ABI5 (Met1-Trp388).
Target bZIP transcription factor ABI5 homolog, OsABI5, bZIP transcription factor 10, OsBZIP10, ABI5, BZIP10, OREB1, Os01g0859300, LOC_Os01g64000, OsJ_04142, P0489B03.11, P0679C12.1
Purification Purified by antigen affinity column.
Accession Q8RZ35
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

bZIP transcription factor ABI5 homolog is a ~41 kDa protein. Transcription factor that possesses transactivation activity in yeast. Involved in abscisic acid (ABA) signaling pathway. Binds to the G-box motif 5'-CACGTG-3' of TRAB1 gene promoter. Involved in the regulation of pollen maturation. May act as negative regulator of salt stress response.

1. Zou, M. et al. (2007) Biochemical and biophysical research communications 360, 307-13. PMID: 17604002
2. Zou, M. et al. (2008) Plant molecular biology 66, 675-83. PMID: 18236009
3. Hong, JY. et al. (2011) Phytochemistry 72, 27-36. PMID: 21055780
4. Kim, H. et al. (2012) Journal of experimental botany 63, 1013-24. PMID: 22071266
Note For research use only.
Images
  • Anti-Rice ABI5 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-Rice ABI5 polyclonal antibody at 1ug/mL on recombinant Rice ABI5 (Catalog No. PR141012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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