Anti-Rice CENH3 Polyclonal Antibody (PR109024)

Name: Anti-Rice CENH3 Polyclonal Antibody
Brand: abinScience
SKU: PR109024
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

PR109024

Description

Anti-Rice CENH3 Polyclonal Antibody (PR109024) is a rabbit polyclonal antibody detecting Histone H3-like centromeric protein CENH3; Centromeric histone CENH3; Centromeric histone H3; CENH3; Os05g0489800; LOC_Os05g41080; OJ1119_H02.19; OsJ_19013 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Oryza sativa (Rice)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant rice CENH3 (Gly71-Trp164).

Target

Histone H3-like centromeric protein CENH3; Centromeric histone CENH3; Centromeric histone H3; CENH3; Os05g0489800; LOC_Os05g41080; OJ1119_H02.19; OsJ_19013

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q6T367

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Histone H3-like centromeric protein CENH3 is a ~18 kDa protein. Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation (Probable).

1. Nagaki, K. et al. (2004) Nature genetics 36, 138-45. PMID: 14716315

2. Yan, H. et al. (2006) The Plant cell 18, 2123-33. PMID: 16877494

Note

For research use only

Images

Western blot

Lane 1: HCT116 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Raji cell lysate
Lane 6: Hela cell lysate
Lane 7: A375 cell lysate
Lane 8: A431 cell lysate
Lane 9: K562 cell lysate

References

Datasheet

Anti-Rice CENH3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Rice CENH3 Polyclonal Antibody (PR109024)

Overview

Images

References

Datasheet

SDS

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