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Anti-RNASE4 Polyclonal Antibody (HW563014)

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Overview
Catalog No.HW563014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human RNASE4 (Gln29-Gly147).
Target RNS4, Ribonuclease 4, RNase 4, RNASE4
Purification Purified by antigen affinity column.
Accession P34096
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ribonuclease 4 (RNASE4) is a ~16 kDa protein. Cleaves preferentially after uridine bases. Has antimicrobial activity against uropathogenic E.coli (UPEC). Probably contributes to urinary tract sterility.

1. Shapiro, R. et al. (1986) Biochemistry 25, 7255-64. PMID: 3467790
2. Bender, K. et al. (2021) American journal of physiology. Renal physiology 320, F972-F983. PMID: 33818125
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-RNASE4 Polyclonal Antibody

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