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Anti-RNF113A Polyclonal Antibody (HB570014)

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Overview
Catalog No.HB570014
Description
Anti-RNF113A Polyclonal Antibody (HB570014) is a rabbit polyclonal antibody detecting E3 ubiquitin-protein ligase RNF113A; 2.3.2.27; Cwc24 homolog; RING finger protein 113A; Zinc finger protein 183; RNF113A; RNF113; ZNF183 in ELISA, IHC, WB. Suitable for Human and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human RNF113A (Ile201-Thr343).
Target E3 ubiquitin-protein ligase RNF113A; 2.3.2.27; Cwc24 homolog; RING finger protein 113A; Zinc finger protein 183; RNF113A; RNF113; ZNF183
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O15541, Q67ER4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

E3 ubiquitin-protein ligase RNF113A is a ~38 kDa protein. Required for pre-mRNA splicing as component of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). E3 ubiquitin-protein ligase that catalyzes the transfer of ubiquitin onto target proteins. Catalyzes polyubiquitination of SNRNP200/BRR2 with non-canonical 'Lys-63'-linked polyubiquitin chains. Plays a role in DNA repair via its role in the synthesis of 'Lys-63'-linked polyubiquitin chains that recruit ALKBH3 and the ASCC complex to sites of DNA damage by alkylating agents.

1. Zhang, X. et al. (2018) Cell research 28, 307-322. PMID: 29360106
2. Haselbach, D. et al. (2018) Cell 172, 454-464.e11. PMID: 29361316
3. Lear, T. et al. (2017) American journal of physiology. Cell physiology 313, C584-C592. PMID: 28978524
4. Brickner, JR. et al. (2017) Nature 551, 389-393. PMID: 29144457
Note For research use only
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