Anti-RNF220 Polyclonal Antibody (HA773014)

Name: Anti-RNF220 Polyclonal Antibody
Brand: abinScience
SKU: HA773014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA773014

Description

Anti-RNF220 Polyclonal Antibody (HA773014) is a rabbit polyclonal antibody detecting E3 ubiquitin-protein ligase RNF220; 2.3.2.27; RING finger protein 220; RING-type E3 ubiquitin transferase RNF220; RNF220; C1orf164 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human RNF220 (Lys482-Leu566).

Target

E3 ubiquitin-protein ligase RNF220; 2.3.2.27; RING finger protein 220; RING-type E3 ubiquitin transferase RNF220; RNF220; C1orf164

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q5VTB9, Q08DV5, Q6PDX6

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase RNF220 is a ~62 kDa protein. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B. Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 promoting Wnt signaling. Plays a critical role in the regulation of nuclear lamina.

1. Ma, P. et al. (2014) Molecular and cellular biology 34, 4355-66. PMID: 25266658

2. Sferra, A. et al. (2021) Brain : a journal of neurology 144, 3020-3035. PMID: 33964137

Note

For research use only

Images

References

Datasheet

Anti-RNF220 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-RNF220 Polyclonal Antibody (HA773014)

Overview

Images

References

Datasheet

SDS