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Anti-RNMT Polyclonal Antibody (HB576014)

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Overview
Catalog No.HB576014
Description
Anti-RNMT Polyclonal Antibody (HB576014) is a rabbit polyclonal antibody detecting mRNA cap guanine-N7 methyltransferase; 2.1.1.56; RG7MT1; mRNA (guanine-N(7))-methyltransferase; mRNA cap methyltransferase; hCMT1; hMet; hcm1p; RNMT; KIAA0398 in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human RNMT (Gly144-Gln476).
Target mRNA cap guanine-N7 methyltransferase; 2.1.1.56; RG7MT1; mRNA (guanine-N(7))-methyltransferase; mRNA cap methyltransferase; hCMT1; hMet; hcm1p; RNMT; KIAA0398
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O43148, Q5U2U7, Q9D0L8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

mRNA cap guanine-N(7) methyltransferase (RNMT) is a ~54 kDa protein. Catalytic subunit of the mRNA-capping methyltransferase RNMT:RAMAC complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.

1. Saha, N. et al. (1999) The Journal of biological chemistry 274, 16553-62. PMID: 10347220
2. Wen, Y. et al. (2000) Genes & development 14, 2944-9. PMID: 11114884
3. Gonatopoulos-Pournatzis, T. et al. (2011) Molecular cell 44, 585-96. PMID: 22099306
4. Varshney, D. et al. (2016) Nucleic acids research 44, 10423-10436. PMID: 27422871
5. Pillutla, RC. et al. (1998) The Journal of biological chemistry 273, 21443-6. PMID: 9705270
6. Tsukamoto, T. et al. (1998) Biochemical and biophysical research communications 251, 27-34. PMID: 9790902
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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Anti-RNMT Polyclonal Antibody

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