Anti-RORC/NR1F3/RORgT Polyclonal Antibody (HW351014)

Name: Anti-RORC/NR1F3/RORgT Polyclonal Antibody
Brand: abinScience
SKU: HW351014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HW351014

Description

Anti-RORC/NR1F3/RORgT Polyclonal Antibody (HW351014) is a rabbit polyclonal antibody detecting NR1F3 in ELISA, IHC, WB. Suitable for Human, Mouse, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Pongo abelii

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RORC/NR1F3/RORgT (Glu212-Ser461).

Target

RAR-related orphan receptor C, RORC, NR1F3, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, Retinoid-related orphan receptor-gamma, RZRG, Nuclear receptor subfamily 1 group F member 3, RORG, RORgT

Purification

Purified by antigen affinity column.

Accession

P51449

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nuclear receptor ROR-gamma (RORC/NR1F3) is a ~58 kDa protein. Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of cellular differentiation, immunity, peripheral circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism. Considered to have intrinsic transcriptional activity, have some natural ligands like oxysterols that act as agonists (25-hydroxycholesterol) or inverse agonists (7-oxygenated sterols), enhancing or repressing the transcriptional activity, respectively. Recruits distinct combinations of cofactors to target gene regulatory regions to modulate their transcriptional expression, depending on the tissue, time and promoter contexts. Regulates the circadian expression of clock genes such as CRY1, BMAL1 and NR1D1 in peripheral tissues and in a tissue-selective manner.

1. Jetten, AM. (2009) Nuclear receptor signaling 7, e003. PMID: 19381306

2. Wang, Y. et al. (2010) The Journal of biological chemistry 285, 5013-25. PMID: 19965867

3. Jin, L. et al. (2010) Molecular endocrinology (Baltimore, Md.) 24, 923-9. PMID: 20203100

4. Solt, LA. et al. (2012) Trends in endocrinology and metabolism: TEM 23, 619-27. PMID: 22789990

5. Okada, S. et al. (2015) Science (New York, N.Y.) 349, 606-613. PMID: 26160376

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RORC/NR1F3/RORgT polyclonal antibody at 1ug/mL on recombinant RORC/NR1F3/RORgT (Catalog No. HW351012).

References

Datasheet

Anti-RORC/NR1F3/RORgT Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-RORC/NR1F3/RORgT Polyclonal Antibody (HW351014)

Overview

Images

References

Datasheet

SDS

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