Anti-RPA1 Polyclonal Antibody (HA686014)

Name: Anti-RPA1 Polyclonal Antibody
Brand: abinScience
SKU: HA686014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA686014

Description

Anti-RPA1 Polyclonal Antibody (HA686014) is a rabbit polyclonal antibody detecting N-terminally processed; RPA1; REPA1; RPA70 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human RPA1 (Ile187-Met616).

Target

Replication protein A 70 kDa DNA-binding subunit; RP-A p70; Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding protein; Replication protein A 70 kDa DNA-binding subunit, N-terminally processed; RPA1; REPA1; RPA70

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P27694, Q8VEE4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Replication protein A 70 kDa DNA-binding subunit (RPA1) is a ~68 kDa protein. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response.

1. Gupta, R. et al. (2007) Blood 110, 2390-8. PMID: 17596542

2. Haahr, P. et al. (2016) Nature cell biology 18, 1196-1207. PMID: 27723717

3. Bass, TE. et al. (2016) Nature cell biology 18, 1185-1195. PMID: 27723720

4. Lin, YL. et al. (1998) The Journal of biological chemistry 273, 1453-61. PMID: 9430682

5. Maréchal, A. et al. (2014) Molecular cell 53, 235-246. PMID: 24332808

6. Sleeth, KM. et al. (2007) Journal of molecular biology 373, 38-47. PMID: 17765923

7. Aboussekhra, A. et al. (1995) Cell 80, 859-68. PMID: 7697716

8. DeMott, MS. et al. (1998) The Journal of biological chemistry 273, 27492-8. PMID: 9765279

9. Grudic, A. et al. (2007) Nucleic acids research 35, 7267-78. PMID: 17959650

10. Sharma, R. et al. (2022) Blood 139, 1039-1051. PMID: 34767620

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-RPA1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Lncap cell lysate

References

Datasheet

Anti-RPA1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-RPA1 Polyclonal Antibody (HA686014)

Overview

Images

References

Datasheet

SDS

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