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Anti-RPL30 Polyclonal Antibody (HB432014)

Anti-RPL30 Polyclonal Antibody
Anti-RPL30 Polyclonal Antibody
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Overview
Catalog No.HB432014
Description
Anti-RPL30 Polyclonal Antibody (HB432014) is a rabbit polyclonal antibody detecting Large ribosomal subunit protein eL30; 60S ribosomal protein L30; RPL30 in ELISA, IHC, WB. Suitable for Rat, Human, Mouse, Bovine, and Chicken.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human RPL30 (Met1-Lys115).
Target Large ribosomal subunit protein eL30; 60S ribosomal protein L30; RPL30
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P62890, P62888, P62889, Q3T0D5, P67883, G1TDL2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Large ribosomal subunit protein eL30 (RPL30) is a ~12 kDa protein. Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

Note For research use only
Images
  • Anti-RPL30 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RPL30 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Jurkat cell lysate
    Lane 2: MCF-7 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: SH-SY5Y cell lysate
    Lane 5: Hela cell lysate
    Lane 6: PC-3 cell lysate
    Lane 7: Rat brain lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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