Anti-RPP30 Polyclonal Antibody (HA739014)

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Datasheet
SDS

Overview

Catalog No.

HA739014

Description

Anti-RPP30 Polyclonal Antibody (HA739014) is a rabbit polyclonal antibody detecting Ribonuclease P protein subunit p30; RNaseP protein p30; RNase P subunit 2; RPP30; RNASEP2 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Bovine, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human RPP30 (Met1-Thr237).

Target

Ribonuclease P protein subunit p30; RNaseP protein p30; RNase P subunit 2; RPP30; RNASEP2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P78346, Q3SZ21, O88796

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ribonuclease P protein subunit p30 (RPP30) is a ~29 kDa protein. Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.

1. Wu, J. et al. (2018) Cell 175, 1393-1404.e11. PMID: 30454648

2. Eder, PS. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 1101-6. PMID: 9037013

3. Jarrous, N. et al. (1998) RNA (New York, N.Y.) 4, 407-17. PMID: 9630247

4. Goldfarb, KC. et al. (2017) Genes & development 31, 59-71. PMID: 28115465

Note

For research use only

Images

References

Datasheet

Anti-RPP30 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-RPP30 Polyclonal Antibody (HA739014)

Overview

Images

References

Datasheet

SDS

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