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Anti-RPS13 Polyclonal Antibody (HA734014)

Anti-RPS13 Polyclonal Antibody
Anti-RPS13 Polyclonal Antibody
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Overview
Catalog No.HA734014
Description
Anti-RPS13 Polyclonal Antibody (HA734014) is a rabbit polyclonal antibody detecting Small ribosomal subunit protein uS15; 40S ribosomal protein S13; RPS13 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Pig, and Chicken.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human RPS13 (Lys27-Ala151).
Target Small ribosomal subunit protein uS15; 40S ribosomal protein S13; RPS13
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q56JX8, P62277, P62301, P62279, Q6ITC7, G1SP51, P62278, P49393, Q03334
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Small ribosomal subunit protein uS15 (RPS13) is a ~17 kDa protein. Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.

Note For research use only
Images
  • Anti-RPS13 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RPS13 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: MCF-7 cell lysate
    Lane 2: U87 cell lystae
    Lane 3: Hela cell lysate
    Lane 4: BxPC-3 cell lysate
    Lane 5: A549 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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