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Anti-RV-A NSP4 Polyclonal Antibody (VK676084)

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Overview
Catalog No.VK676084
Description
Anti-RV-A NSP4 Polyclonal Antibody (VK676084) is a rabbit polyclonal antibody detecting NSP4 in ELISA, IHC, WB. Suitable for Rotavirus A.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityRotavirus A (RV-A)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant RV-A NSP4 (Leu43-Asp143).
Target NSP4, NSP4
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession B3SRT2, Q9PYC7, B3SRQ8, P08434, Q9PYD2, Q6YLV5, P89059, Q9PYD1, A2T3Q0, P30031, Q9PYD0, Q82030, Q82028, Q9PYC8, P04512, Q3ZK64, P89063, P04513, Q82029, Q91E93, P30030, Q77Q91, O56850, Q6QT02, Q8JNB2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Non-structural glycoprotein 4 (NSP4) is a ~20 kDa protein. Plays an essential role in the virus replication cycle by acting as a viroporin. Creates a pore in the host endoplasmic reticulum and as a consequence releases Ca(2+) in the cytoplasm of infected cell. In turn, high levels of cytoplasmic calcium trigger membrane trafficking and transport of viral ER-associated proteins to viroplasms, sites of viral genome replication and immature particle assembly.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-RV-A NSP4 Polyclonal Antibody

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