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Anti-RV-A VP3 Polyclonal Antibody (VK676064)

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Overview
Catalog No.VK676064
Description
Anti-RV-A VP3 Polyclonal Antibody (VK676064) is a rabbit polyclonal antibody detecting VP3 in ELISA, IHC, WB. Suitable for Rotavirus A.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityRotavirus A (RV-A)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant RV-A VP3 (Gly530-Glu835).
Target VP3, VP3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6WNW5, B1NKS7, B2BMF9, A2T3S5, B1NKR1, A4ZCW6, A7J3A0, Q6WNW3, Q8BB04, P15736, B3F2X7, Q3ZK57, A7J392
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein VP3 is a ~97 kDa protein. Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-RV-A VP3 Polyclonal Antibody

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