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Anti-RV-A VP6 Polyclonal Antibody (VK021014)

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Overview
Catalog No.VK021014
Description
Anti-RV-A VP6 Polyclonal Antibody (VK021014) is a rabbit polyclonal antibody detecting Intermediate capsid protein VP6 in ELISA, IHC, WB. Suitable for Rotavirus A.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityRotavirus A (RV-A)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant RV-A VP6 (Met1-Lys397).
Target Intermediate capsid protein VP6
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q86219, A7J3A1, A7J3A5, P03531, B1NKU0, B1NKS8, Q86347, B1NKU4, P16488, A4ZCW7, P03530, Q86345, Q91N56, B1NKQ8, P16593, P16592, B1NKR2, P04509, Q06386, B1NKR6, B1NKT2, Q89601, Q9QNB0, P89043, P16487, P18610, A2T3T0, Q3ZK59, P87723, A7J3A8, Q8JTI5, P08035, Q6PMI4, Q6LE89, B2BN53, Q91N61
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Intermediate capsid protein VP6 is a ~44 kDa protein. Intermediate capsid protein that self assembles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. This capsid constitutes the middle concentric layer of the viral mature particle. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nascent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP6 is required for the transcription activity of the DLP.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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