Anti-S100A1 Polyclonal Antibody (HB172014)

Name: Anti-S100A1 Polyclonal Antibody
Brand: abinScience
SKU: HB172014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HB172014

Description

Anti-S100A1 Polyclonal Antibody (HB172014) is a rabbit polyclonal antibody detecting S100A1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human S100A1 (Met1-Ser94).

Target

S100A1, Protein S100-A1, S-100 protein alpha chain, S100 calcium-binding protein A1, S100A, S-100 protein subunit alpha

Purification

Purified by antigen affinity column.

Accession

P23297

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein S100-A1 (S100A1) is a ~10 kDa protein. Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation. In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes. These changes allow interactions with specific target proteins and modulate their activity. Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase. Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole.

1. Kiewitz, R. et al. (2003) Biochemical and biophysical research communications 306, 550-7. PMID: 12804600

2. Nowakowski, M. et al. (2013) Biochemistry 52, 1149-59. PMID: 23351007

3. Yamaguchi, F. et al. (2012) The Journal of biological chemistry 287, 13787-98. PMID: 22399290

4. Most, P. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 13889-94. PMID: 11717446

Note

For research use only.

Images

References

Datasheet

Anti-S100A1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-S100A1 Polyclonal Antibody (HB172014)

Overview

Images

References

Datasheet

SDS

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