Anti-S100A12 Polyclonal Antibody (HX203014)

Name: Anti-S100A12 Polyclonal Antibody
Brand: abinScience
SKU: HX203014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX203014

Description

Anti-S100A12 Polyclonal Antibody (HX203014) is a rabbit polyclonal antibody detecting S100A12 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human S100A12 (Met1-Glu92).

Target

Migration inhibitory factor-related protein 6, MRP-6, Extracellular newly identified RAGE-binding protein, p6, Calcium-binding protein in amniotic fluid 1, Calgranulin-C, Protein S100-A12, CGRP, S100 calcium-binding protein A12, Neutrophil S100 protein, CAAF1, EN-RAGE, S100A12, CAGC

Purification

Purified by antigen affinity column.

Accession

P80511

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein S100-A12 (S100A12) is a ~10 kDa protein. S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its pro-inflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of pro-inflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-S100A12 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Human blood lysate
Lane 2: Human blood lysate
Lane 3: Mouse lung lysate
Lane 4: Mouse liver lysate
Lane 5: Mouse spleen lysate
Lane 6: Rat lung lysate
Lane 7: Rat liver lysate
Lane 8: Rat spleen lysate

References

Datasheet

Anti-S100A12 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-S100A12 Polyclonal Antibody (HX203014)

Overview

Images

References

Datasheet

SDS

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