Anti-S6K1/RPS6KB1 Polyclonal Antibody (HB044014)

Name: Anti-S6K1/RPS6KB1 Polyclonal Antibody
Brand: abinScience
SKU: HB044014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HB044014

Description

Anti-S6K1/RPS6KB1 Polyclonal Antibody (HB044014) is a rabbit polyclonal antibody detecting RPS6KB1 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human S6K1/RPS6KB1 (Phe91-Phe352).

Target

S6K-beta-1, 70 kDa ribosomal protein S6 kinase 1, p70 S6KA, p70-S6K 1, S6K1, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, STK14A, RPS6KB1, Ribosomal protein S6 kinase I, p70 S6K-alpha, P70S6K1, Ribosomal protein S6 kinase beta-1

Purification

Purified by antigen affinity column.

Accession

P23443

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ribosomal protein S6 kinase beta-1 (S6K1/RPS6KB1) is a ~59 kDa protein. Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mechanistic target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B.

1. Wang, X. et al. (2001) The EMBO journal 20, 4370-9. PMID: 11500364

2. Fleckenstein, DS. et al. (2003) Leukemia research 27, 687-94. PMID: 12801526

3. Fingar, DC. et al. (2004) Molecular and cellular biology 24, 200-16. PMID: 14673156

4. Raught, B. et al. (2004) The EMBO journal 23, 1761-9. PMID: 15071500

5. Richardson, CJ. et al. (2004) Current biology : CB 14, 1540-9. PMID: 15341740

6. Holz, MK. et al. (2005) Cell 123, 569-80. PMID: 16286006

7. Zhang, HH. et al. (2006) Molecular cell 24, 185-97. PMID: 17052453

8. Dorrello, NV. et al. (2006) Science (New York, N.Y.) 314, 467-71. PMID: 17053147

9. Djouder, N. et al. (2007) Molecular cell 28, 28-40. PMID: 17936702

10. Zhang, J. et al. (2008) The Journal of biological chemistry 283, 35375-82. PMID: 18952604

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-S6K1/RPS6KB1 polyclonal antibody at 1ug/mL on recombinant S6K1/RPS6KB1 (Catalog No. HB044012).
Western blot

Western blot analysis was performed using anti-RPS6KB1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate

References

Datasheet

Anti-S6K1/RPS6KB1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-S6K1/RPS6KB1 Polyclonal Antibody (HB044014)

Overview

Images

References

Datasheet

SDS

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